Fig 1: ADAMTS2, COL12A1 and THBS2 are the direct target genes of m6A RNA demethylase FTO. (A) qRT-PCR showed that the mRNA levels of ADAMTS2, COL12A1, and THBS2 in siFTO Panc-1 cell line were significantly down-regulated compared with the si-NC group. (B) Dot blot showed that m6A level of pancreatic cancer cells was increased after knocking down FTO. (C) The SRAMP database predicted that m6A modification sites of ADAMTS2, COL12A1, and THBS2. (D) Motifs and metagene analyses from RMbase database revealed the m6A motifs enrichment and modification peak of ADAMTS2, COL12A1, and THBS2. (E) MeRIP analysis indicated that FTO knockdown remarkably increased the m6A level of ADAMTS2, COL12A1, and THBS2 mRNA. *p < 0.05, **p < 0.01, ***p < 0.001.